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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
| Hematology | 20–25% | - Physiology and disease states - Laboratory testing and hemostasis |
| Immunology | 5–10% | - Principles of immunology - Serologic procedures and immune diseases |
| Urinalysis and Other Body Fluids | 5–10% | - Physical and chemical testing - Microscopic analysis and disease correlation |
| Chemistry | 20–25% | - Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology |
| Blood Banking | 15–20% | - Blood group systems and immunology - Blood products and components - Transfusion practice and testing |
| Microbiology | 15–20% | - Bacteriology and mycology procedures - Virology and parasitology testing |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. The area of the federal government that ensures that the laboratory is stocked with gloves for their employees when performing tests on patient blood sample is:
A) HIPAA.
B) CDC.
C) FDA.
D) OSHA
2. Failure to deliver glucose drawn in a serum separator tube (SST) to the laboratory within the recommended time will cause:
Question options:
A) glycolysis
B) a falsely decreased glucose value
C) a falsely increased glucose value
D) both A and C
3. The Westgard multi-rule 22S describes the scenario where two consecutive data points fall outside +2SD or
-2SD. If this occurs, then the run must be rejected. This situation is most likely caused by a systematic error.
Which of the following describes the Westgard multi-rule 22S?
A) Two consecutive data points fall outside +2SD or -2SD
B) Two control data points are within ± 2s
C) One control data point falls outside +2s and a second point falls outside - 2s
D) Two points are within 2SD
E) Two consecutive data points fall inside +2s
4. S. aureus does not have capsular material as a defense mechanism. H. influenzae, K. pneumoniae, N.
meningitidis, and S. pneumoniae each have capsular material use as a defense mechanism. This capsular material is an antiphagocytic substance which helps deter bacterial death by macrophages and other white blood cells.
Microbiology
Which one of the following does NOT have capsular material as a defense mechanism?
A) S. pneumoniae
B) H. influenzae
C) S. aureus
D) N. meningitidis
E) K. pneumoniae
5. The Needlestick Safety and Prevention Act requires employers to:
A) test MLTs annually for hepatitis B and HIV
B) involve MLTs in the selection of safety devices
C) allow MLTs to work flexible schedules
D) provide MLTs with plastic capillary tubes
Solutions:
| Question # 1 Answer: D | Question # 2 Answer: D | Question # 3 Answer: A | Question # 4 Answer: A | Question # 5 Answer: B |







